BrDMC1, a Recombinase Gene, Is Involved in Seed Germination in Brassica rapa under Salt Stress

Recombinases are in part responsible for homologous recombination and genome integrity during DNA repair. DMC1 has a typical RecA domain, belongs to the recombinase superfamily. The reactive oxygen species (ROS) as potent damage agent is produced seed germination under stress conditions. repair should be initiated immediately allow subsequent seedling development. In this study, we attempted characterize underlying mechanism of BrDMC1 responsiveness salinity using RNA interference approach Brassica rapa (B. rapa). Bioinformatics expression pattern analysis revealed that only retained BrDMC1.A01 after whole triplication (WGT) event was primarily transcribed flowers seeds. had high activity promoter region germination, according histochemical GUS staining. data showed salt treatment reduced rate, weakened vigor decreased antioxidant enzyme activity, but increased oxidative BrDMC1-RNAi Furthermore, stress-responsive genes significantly different transgenic lines exposed stress. Therefore, may respond by controlling stress-related B. rapa.